卢 嘉 锡 讲 座
题 目: Capturing the intermediate state of riboswitch-ligand interaction using X-ray free electron laser
讲座人: 王运星 资深研究员
Director, NCI Small Angle X-ray Scattering Center
(Cancer Institute, National Institutes of Health, USA)
时 间: 4月10日（周一）上午10:00-12:00
地 点: 卢嘉锡楼202报告厅
欢迎各位老师同学积极参加！
嘉宾介绍：
王运星博士是国际知名的结构生物学专家，现为美国NIH癌症研究所（NCI）小角散射中心主任, 主要从事RNA、蛋白质及其复合物的结构、功能及其方法学研究。他已在Cell, Nature，Nat. Chem. Biol.，Mol. Cell, Curr. Opn. Str. Biol. 和JACS等国际刊物上发表了100多篇研究论文，参与编著3部RNA和生物核磁相关的专著。他于2014、2016年分别获得NIH“Outstanding Basic Science Award”及“NIH Director Award for Outstanding Achievement in Medical and Science”奖。
报告摘要：
In the genetic regulation by riboswitch RNAs, ligand binding to the aptamer domain triggers a signal to the downstream expression platform. A complete understanding of the structural basis for this mechanism requires the ability to study structural changes over time. With the application of serial femtosecond crystallography (SFX) and an X-ray free electron laser (XFEL), advances have been made in both time-resolved crystallography and riboswitch biology. A ligand-triggered real time crystallography has led to structure determination of the adenine riboswitch aptamer domain during the course of a four-state interaction involving two apo, an intermediate, and the final bound states. These structures serve as the strong proof of the four-state reaction kinetics model and illustrate the structural basis for signal transmission. Moreover, a polymorphic phase transition was observed when the RNA molecules were fully converted to the bound state in crystals. These results demonstrate the utility of ligand-triggered real time crystallography to study structural transitions in crystallo, including those involving large conformational changes. General implications of ligand-triggered real time crystallography will also be discussed.
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化学生物学系生物核磁课题组
