学术报告：Rapid Detection of Single Bacteria in Unprocessed Blood using Integrated Comprehensive Droplet Digital Detection

厦门大学谱学分析与仪器教育部重点实验室
biwn必赢
2016年11月16日

报告摘要：

Rapid Detection of Single Bacteria in Unprocessed Blood using Integrated Comprehensive Droplet Digital Detection 

Dong-Ku Kang*

Department of Chemistry, Incheon National University

^*E-mail: dkkang@inu.ac.kr

Antimicrobial resistance is a growing health problem in the United States and worldwide. According to the Centers for Disease Control and Prevention (CDC), more than two million people are infected annually with antibiotic-resistant infections, with >23,000 deaths. Aggressive bacterial infections associated with antimicrobial resistance are often managed within intensive care units (ICUs) with high associated costs, which impose significant healthcare, economic and social burdens. Especially, extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is commonly found in K. pneumoniae and Escherichia coli are among the most prevalent antimicrobial resistant pathogens. ESBLs account for 10-20% of serious E. coli infections and 20-30% of Klebsiella infections reported nationally. However lack of rapid diagnostics results in either use of unnecessarily broad empiric antibiotics, or a delay of several days in administering the appropriate antibiotic(s).

Rapid diagnostics are particularly needed for pathogens such as E. coli, which are common, virulent, and have acquired ESBLs1. Furthermore, diagnostic tests that can confirm the presence of ESBLs regardless of the species would be exceedingly valuable in directing early therapy and enabling better antimicrobial stewardship for those not infected with antibiotic resistant pathogens. Unfortunately, existing bacterial detection methods are limited in their inability to rapidly detect and identify pathogens that typically occur at low concentrations in blood (1 to 100 colony-forming unit (CFU)/mL) as is commonly found in adult BSIs. Conventional bacterial blood cultures coupled with susceptibility testing (automated methods or disk diffusion) require days to obtain a result. This lag in time to detect a patient with a culture positive BSI, identification of the isolate and establishing the antimicrobial susceptibility of the isolate contribute to the high mortality.

Here, we will discuss about our strategy for monitoring beta-lactamase producing bacteria at single-cell sensitivity within a few hours by miniaturized droplet-based microfluidic system. 

*Keywords: Lab on a chip, Microdroplet, Microfluidics, Digital quantification

附件1：报告摘要
附件2：报告人简历