报告题目：Science of Rock ’n’ Roll
- Single Molecule and Nanoparticle Imaging in Biophysical, Surface,
and Photocatalysis Studies
报告人： Prof. Ning Fang
Ames Laboratory, U.S. Department of Energy, USA
Iowa State University, USA
时间： 5月16日(周四) 上午10:00
地点： 卢嘉锡楼报告厅（202）
以下为报告的摘要部分：
Science of Rock ’n’ Roll
- Single Molecule and Nanoparticle Imaging in Biophysical, Surface, and Photocatalysis Studies
Ning Fang
Ames Laboratory, U.S. Department of Energy and Iowa State University, Ames, Iowa 50011
报告时间：5月16日（周四） 10:00am
报告地点：卢嘉锡楼报告厅（202）
Abstract:
The research in the Fang Laboratory aims to open up new frontiers in chemical and biological discovery through the development and use of novel optical imaging platforms, which provide sub-diffraction-limited spatial resolution, high angular resolution, excellent detectability, and/or nanometer localization precision for single molecules and nanoparticles.
• Rotational Tracking: The knowledge of rotational dynamics in and on live cells remains highly limited due to technical limitations. The single particle orientation and rotational tracking (SPORT) technique has been developed for visualizing rotational motions of anisotropic plasmonic gold nanorods under a differential interference contrast (DIC) microscope. The SPORT technique is capable of extracting important information (including rotational rates, modes, and directions) on the characteristic rotational dynamics involved in complex biological processes, such as endocytosis and intracellular transport.
• Fluorescence Super-Resolution and High-Precision Tracking: A fully automatic calibration and scanning prism-type total internal reflection fluorescence (TIRF) and scattering (TIRS) microscope has been constructed for high-precision tracking of single molecules and nanoparticles. Stable signals from non-blinking quantum dots under the automated TIRF microscope allows for continuous super-localization of single quantum dots with sub-10-nm precision in both lateral and axial directions. This system is also being used to reveal the nature and photocatalytic properties of the surface active sites on single Au-CdS hybrid nanocatalysts.
Selected publications:
• Single Cell Optical Imaging and Spectroscopy. Chem. Rev. 2013, Article ASAP. [Online ]
• Three-dimensional High-resolution Rotational Tracking with Super-localization Reveals Conformations of Surface-bound Anisotropic Nanoparticles. Nano Lett. 2013, 13(3), 1245-1250. [Online ]
• Rotational Dynamics of Cargos at Pauses during Axonal Transport. Nat. Commun. 2012, 3:1030, doi: 10.1038/ncomms2037. [Online ]
• Focused Orientation and Position Imaging (FOPI) of Single Anisotropic Plasmonic Nanoparticles by Total Internal Reflection Scattering Microscopy. Nano Lett. 2012, 12(8), 4282-4288. [Online ]
• High-Precision Tracking with Non-Blinking Quantum Dots Resolves Nanoscale Vertical Displacement. J. Am. Chem. Soc. 2012, 134(14), 6108-6111. [Online ]
• Determining Full Three-Dimensional Orientation of Single Anisotropic Nanoparticles by Differential Interference Contrast Microscopy. Angew. Chem. Int. Ed. 2012, 51(31), 7734-7738. [Online ]
• Single Particle Orientation and Rotation Tracking Discloses Distinctive Rotational Dynamics of Drug Delivery Vectors on Live Cell Membranes. J. Am. Chem. Soc. 2011, 133(15), 5720-5723. [Online ]
• Resolving Rotational Motions of Nano-objects in Engineered Environments and Live Cells with Gold Nanorod Probes and Differential Interference Contrast Microscopy. J. Am. Chem. Soc. 2010, 132 (46), 16417-16422. [Online ]
• Auto-Calibrated Scanning-Angle Prism-Type Total Internal Reflection Florescence Microscopy for Nanometer-Precision Axial Position Determination. Anal. Chem. 2010, 82(6), 2441-2447. [Online ]
欢迎感兴趣的老师与学生参加，谢谢 ！
固体表面物理化学国家重点实验室
biwn必赢
2013-05-15
