报告题目：Optical Imaging of Nanoscale Chemical and Biological Processes
报告人：方宁 副教授
佐治亚州立大学化学系
讲座时间：2019.5.31（星期五）下午 14:30
讲座地点：卢嘉锡楼202报告厅
报告摘要：
The research in the Fang Laboratory aims to open new frontiers in chemical and biological discovery through the development and use of novel optical imaging platforms, which provide sub-diffraction-limited spatial resolution, high angular resolution (for anisotropic imaging probes), excellent detectability, and/or nanometer localization precision for single molecules and nanoparticles.
Rotational Tracking: The knowledge of rotational dynamics in and on live cells remains highly limited due to technical limitations. The Single Particle Orientation and Rotational Tracking (SPORT) techniques have been developed in the Fang Laboratory to acquire accurate measurements of anisotropic plasmonic gold nanorods in complex cellular environments. Rich information in five dimensions, including the x, y, z coordinates and the two orientation angles (azimuthal angle j and polar angle q , as defined in the figure) of the probe’s transition dipole, can be obtained from SPORT experiments. The SPORT technique is capable of extracting important information (including rotational rates, modes, and directions) on the characteristic rotational dynamics involved in cellular processes, such as adhesion, endocytosis, and transport of functionalized nanoparticles, as may be relevant to drug delivery and viral entry.
Single Molecule Catalysis: Real time imaging of single catalyst active sites in situ enables mechanistic studies on fundamental reaction steps under actual turnover operando conditions; these studies have enormous potential impact in establishing intimate structure-property relationships from which to build better (faster, cleaner, cheaper) catalysts. Our research aims to design catalytic platforms for single molecule imaging and reveal molecular dynamics (including diffusion, adsorption, and chemical conversion, as well as their coupling) on the nanocatalyst surfaces or in the nanoporous structures at the single-molecule level.
报告人简介：
方宁，佐治亚州立大学化学系副教授。1998年厦门大学化学本科，2006年不列颠哥伦比亚大学博士，2006-2008年爱荷华州立大学博士后，2008年就职于爱荷华州立大学化学系助理教授，2015至今就职于佐治亚州立大学副教授。方宁博士的研究方向为新型单分子荧光成像方法和单纳米颗粒微分干涉差成像方法的开发及其在生物化学、表面化学、物理化学和新能源开发领域的应用。在国际上首创了探测纳米棒旋转和定位的SPORT成像技术，并实现了单个荧光分子的超级分辨率成像。代表工作有：Nano Today 2019, 24, 120; Nat. Catal. 2018, 1, 135; Nat. Commun. 2017, 8, 887; PNAS 2017, 114, E5655; Chem. Rev. 2017, 117, 7510; Angew. Chem. Int. Ed. 2014, 53, 12865; J. Am. Chem. Soc. 2014, 136, 1398; Chem. Rev. 2013, 113, 2469; Nano Lett. 2013, 13, 5414; Nano Lett.2013, 13, 1245; Nat. Commun. 2012, 3, 1030; Nano Lett. 2012, 12, 4282; J. Am. Chem. Soc. 2012, 134, 6108; Angew. Chem. Int. Ed. 2012, 51, 7734; J. Am. Chem. Soc. 2011, 133, 5720; J. Am. Chem. Soc. 2010, 132, 16417。
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2019 年 5 月 21 日
